Suppression of VEGF by Aminoguanidine in RPE Cells Cultured in the Hyperglycemic Condition.

Kim, Jae Soon; Lee, In Gul; Cho, Ho Kyun

Original Article

Journal of the Korean Ophthalmological Society 2004;45(2):296-302.
Published online February 1, 2004.

Suppression of VEGF by Aminoguanidine in RPE Cells Cultured in the Hyperglycemic Condition.

Jae Soon Kim, In Gul Lee, Ho Kyun Cho

Department of Ophthalmology, Chung-Ang University College of Medicine, Korea. hkcho26@cau.ac.kr

고혈당 배지에 배양한 망막색소상피세포에서 Aminoguanidine의 VEGF 억제

김재순 ( Kim Jae Sun ) , 이인걸 ( Lee In Geol ) , 조호균 ( Jo Ho Gyun )

Abstract

PURPOSE
We evaluated whether aminoguanidine could inhibit VEGF mRNA expression in the retinal pigment epithelial cells cultured at various glucose concentrations. METHODS: Human retinal pigment epithelial cells were cultured in the culture media containing 5.5 mM, or 11 mM, or 16 mM glucose for 5 days, or 7 days, or 14 days respectively. To evaluate an inhibitory effect of aminoguanidine on VEGF mRNA expression, 1 micro M, or 3 micro M, or 10 micro M aminoguanidine was added in the culture media. The VEGF mRNA expression was assayed by northern blot analysis. RESULTS: The VEGF mRNA expression of the cultured retinal pigment epithelial cells increased proportionally with media glucose concentration in culture media. At each glucose concentration of the media, VEGF mRNA expression increased with a prolongation of incubation period. An aminoguanidine inhibited the expression of VEGF mRNA by concentration-dependent manner in 5 day and 7 day incubation, but not in 14 day incubation. CONCLUSIONS: The aminoguanidine could inhibit a new vessel formation in the diabetic retina, and be useful for therapeutic or preventive drug in the diabetic retinopathy.

Key Words: Aminoguanidine;Hyperglycemia;RPE;VEGF