Effect of Prostaglandin F2alpha on the Production of Nitric Oxide in Cultured Ciliary Muscle Cells.

Kim, Jae Woo; Kim, Yoon Young; Kim, Keun Hae

Original Article

Journal of the Korean Ophthalmological Society 2005;46(1):150-155.
Published online January 31, 2005.

Effect of Prostaglandin F2alpha on the Production of Nitric Oxide in Cultured Ciliary Muscle Cells.

Jae Woo Kim, Yoon Young Kim, Keun Hae Kim

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr

프로스타글란딘F2α가 배양된 섬모체근세포의 산화질소 생성에 미치는 영향

김재우,김윤영,김근해

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea

Correspondence: Jae Woo Kim, M.D.

Abstract

PURPOSE
To investigate whether prostaglandin F2alpha (PGF2alpha) affects on the production of nitric oxide (NO) in cultured ciliary muscle (CM) cells. METHODS: Following primary culture of CM cells from porcine eyes, the cells were exposed to PGF2alpha (PhXA85, latanoprost free acid) with and without pretreatments of NO synthase inhibitor (L-NAME, N omega- Nitro-L-arginine methyl ester) and cyclooxygenase inhibitors (indomethacin and dexamethasone) for 3 days. The cellular survivals were also evaluated in serum-deprived and hypoxic conditions induced by sodium cyanide. The cellular survival and nitrite production were assessed by MTT and Griess assay, respectively. RESULTS: PGF2alpha enhanced the production of NO significantly in cultured CM cells in a dose-dependent manner, while various inhibitors abolished this effect. PGF2alpha was not cytoprotective in serum-deprived and hypoxic conditions. CONCLUSIONS: The current results suggest that PGF2alpha potentiates NO production but is not cytoprotective in CM cells. This PGF2alpha-induced NO production in CM cells may be involved in the regulation of uveoscleral outflow.

Key Words: Ciliary muscle cells;Nitric oxide;PGF2alpha