| Inhibitory Effect of Gefitinib on the Proliferation of Lens Epithelial Cells. |
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Seok Jae Yang, Tae Hwan Kim, Moon Joo Lee, Hyun Kyu Shin, Jung Hyub Oh |
1Department of Ophthalmology, Inha University College of Medicine, 1 Inchon, Korea. jhoh9707@hanmail.net
2Lee Eye Clinic, Guri, Korea.
3Sungnam St. Mary's Eye Clinic, Sungnam, Korea. |
| 게피티니브의 수정체상피세포에 대한 증식억제 효과 |
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양석재1,김태환1,이문주2,신현규3,오중협1 |
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Department of Ophthalmology, Inha University College of Medicine,1 Inchon, Korea, Lee Eye Clinic,2 Guri, Korea, Sungnam St. Mary’s Eye Clinic,3 Sungnam, Korea |
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Correspondence:
Seok-Jae Yang, M.D.1 |
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| Abstract |
PURPOSE
To evaluate the effect of Gefitinib used for the treatment of non-small cell lung cancer on the proliferation of the lens epithelial cells and the activities of growth factors. METHODS: The cell samples were divided into a control group cultured in DMEM and three experimental groups. Group I was exposed to Gefitinib for 3 minutes; group II was exposed to growth factors; and for group III growth factors were added to each concentration of Gefitinib. MMT assays, BrdU staining and morphologically observations with a phase-contrast microscope were used to verify the degrees of cell proliferation. Western blot analysis was conducted to confirm the effects of Gefitinib on extracellular signal-regulated kinase phosphorylation and on type I collagen production. RESULTS: Lens epithelial cell proliferation in experimental group I was reduced to 76% and 56% for 1.00 micro M and 10.00 micro M of Gefitinib, respectively. Experimental group II showed a 140% increase in cell proliferation with EGF treatment. Experimental group III exhibited decreased lens epithelial cell proliferation with both EGF (86% and 66%), and TGF-beta2 (78% and 59%). BrdU staining demonstrated a significant decrease in cell proliferation in groups I and III exposed to more than 1.0 micro M Gefitinib, while group II showed an increase compared to the control group. Moreover, Western blot analysis revealed inhibiting effects on ERK phosphorylation and type I collagen production. CONCLUSIONS: In the culture of human lens epithelial cells, Gefitinib inhibited cell proliferation when used at a concentration above 1.00 (M for 3 minutes. Furthermore, the effects of EGF and TGF-beta2 were also inhibited. |
| Key Words:
EGF;Gefitinib;Lens epithelial cells;TGF-beta2 |
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