Effects of Glutathione on Reactive Oxygen Species-Induced Cytotoxicity in Human Retinal Pigment Epithelial Cell Line.

Koh, Jae Woong; Lee, Byoung Rai; Ji, Nam Chul

Original Article

Journal of the Korean Ophthalmological Society 2002;43(9):1774-1780.
Published online September 1, 2002.

Effects of Glutathione on Reactive Oxygen Species-Induced Cytotoxicity in Human Retinal Pigment Epithelial Cell Line.

Jae Woong Koh, Byoung Rai Lee, Nam Chul Ji

¹Department of Ophthalmology, Chosun University Medical School, Korea. ncchi@mail.chosun.ac.kr
²Department of Biochemistry, Chosun University Medical School, Korea.

인간 망막색소상피세포주에서 반응성산소에 의한 세포손상에 미치는 글루타치온의 영향

고재웅 ( Jae Woong Koh ) , 이병래 ( Byoung Rai Lee ) , 지남철 ( Nam Chul Ji )

Abstract

PURPOSE
Oxidative stress is the well known causative factor for retinal damage. This study investigated the effects of glutathione on reactive oxygen species(ROS) induced injury in human retinal pigment epithelial(HRPE) cells. OBJECTS AND METHODS: HRPE cells (ATCC:CRL-2302) were cultured with DMEM media and exposed to oxidative stress (paraquat, hydrogen peroxide) and/or glutathione modulator[(buthionine sulfoximine (BSO), glutathione (GSH), 2-oxo 4-thiazolidine carboxylic acid (OTC)] for 2 days. The cell viability was determined by measuring the amount of reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT). RESULTS: The rate of MTT reduction of HRPE cells decreased by either paraquat or hydrogen peroxide treatment. BSO as a inhibitor of glutathione biosynthesis potentiated paraquat- or hydrogen peroxideinduced HRPE cells injury. On the other hand GSH or OTC reduced the rate of decrement of MTT reduction in HRPE cells by paraquat and hydrogen peroxide. CONCLUSIONS: Glutathione seemed to play some role in prevention of ROS-induced HRPE cells injury and OTC may be used as an agents for prevention of free radical induced HRPE cell injury.

Key Words: Glutathione;Human retinal pigment epithelial cell;Paraquat;Hydrogen peroxide