| The effect of Hydrogen Peroxide and Ascorbic Acid on Proliferation and Morphology of Lens Epithelial Cells. |
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Jong Hyuck Lee, Jung Hyub Oh, Jung Gon Cho, Sug Jae Kang |
1Department of Ophthalmology, College of Medicine Inha University, Korea. jhoh9707@hanmail.net
2Department of Ophthalmology, Wonju Christian Hospital Wonju College of Medicine Yonsei University, Korea. |
| 수정체 상피의 증식과 변형에 관한 Hydrogen peroxide와 Ascorbic acid의 상호작용 |
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이종혁 ( Jong Hyuck Lee ) , 조정곤 ( Jung Gon Cho ) , 강석재 ( Sug Jae Kang ) , 오중협 ( Jung Hyub Oh ) |
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| Abstract |
PURPOSE
The therapeutic effects of amniotic membrane transplantation (AMT) contain anti-fibrosis, anti-inflammation and facilitation of epithelialization. Thus we investigated whether applying of amniotic membrane (AM) ointment could get the same effect as AMT. METHODS: Alkali burn was induced by applying 6 mm round filter paper which was soaked with 1 N NaOH, onto the central cornea for 60 seconds on both eyes of 10 white rabbits. Then we applied AM ointment on one eye and base ointment on the other eye, four times a day for 1 week. The corneas were harvested for histopathologic studies at 1 day and 3 weeks. Corneal opacity and thickness were measured in 3 days and 1, 2, 3 weeks. RESULTS: In the AM ointment applicated group, the number of the infiltrated PMNs (polymorp-honuclear cells) and apoptotic keratocytes were significantly less than that of the control group (P<0.05). The degree of lipid peroxidation and myofibroblast differentiation were less than those of the control group. Corneal opacity and corneal edema were less in AM ointment group than control group. CONCLUSIONS: AM ointment application after alkali burn is beneficial to reduce inflammation, keratocyte apoptosis and lipid peroxidation, and is considered to suppress corneal haze by these effects. Therefore, this report may be a basic study for the AM ointment research to treat recalcitrant keratitis. |
| Key Words:
Ascorbic acid;Cataract;Fibronectin;Hydrogen peroxide;Lens epithelial cell |
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