The changes of fibronectin in the cultured porcine lens epithelial cells.

Hong, Sung Bum; Lee, Jae Hwan; Kim, Myung Jin; Oh, Jung Hyub

Original Article

Journal of the Korean Ophthalmological Society 2003;44(1):195-201.
Published online January 1, 2003.

The changes of fibronectin in the cultured porcine lens epithelial cells.

Sung Bum Hong, Jae Hwan Lee, Myung Jin Kim, Jung Hyub Oh

¹Department of Ophthalmology, Medical College, Inha University, Korea.
²Bupyung Yonsei Eye Clinic, Korea.

배양된 돼지수정체 상피세포에서의 fibronectin 변화

홍성범 ( Sung Bum Hong ) , 이재환 ( Jae Hwan Lee ) , 김명진 ( Myung Jin Kim ) , 오중협 ( Jung Hyub Oh )

Abstract

PURPOSE
to investigate change of fibronectin as a relating factor of cellular migration in the cultured lens epithelial cells. METHODS: We attempted to observe the fibronectin from cornea, crystalline lens and cultured lens epithelial cells by immunocytochemistry. We also compared the expression of fibronectin from normal porcine crystalline lens epithelial cells and cultured lens epithelial cells that received bFGF with western blot method. RESULTS: Fibronectin was detected in the extracellular matrix of normal corneal epithelium by immunocytochemistry, but not in the normal lens epithelial cells. However, fibronectin was detected in the cytoplasm of the cultured lens epithelial cells and treated with bFGF by immunocytochemistry. CONCLUSIONS: lens epithelial cells in normal condition may not produce fibronectin, but lens epithelial cells without lens nucleus after cataract surgery produce fibronectin.

Key Words: bFGF (basic fibroblast growth factor);Cataract;Fibronectin;Lens epithelial cells