| Usefulness of Newly Developed Film Form Drug Delivery System that Inhibits Lens Epithelial Cell Proliferation. |
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Hyun Woo Kim, Seok Jong Lee, Choun Ki Joo |
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Laboratory of Ophthalmology and Visual Science, College of Medicine, The Catholic university of Korea, Seoul, Korea. ckjoo@catholic.ac.kr |
| 수정체 상피세포 증식억제를 위한 필름 형태의 약물전달 시스템의 유용성 |
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김현우 ( Hyun Woo Kim ) , 이석종 ( Seok Jong Lee ) , 주천기 ( Choun Ki Joo ) |
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| Abstract |
PURPOSE
To develop a therapeutic drug delivery system which inhibits lens epithelial cells proliferation. METHODS: Chitosan, a natural polymer, which is biocompatible and biodegradable was mixed with glycerol with a certain ratio to make it an asceptic film form. Water content and degradability of this film was determined. During the construction of film, 5-fluorouracil (5FU) was added to make of film containing 5FU. Drug release was checked with high performance liquid chromatography (HPLC). Cell cytotoxicity of this film was determined with MTT (3-(4, 5-dimethylathiazol-2yl)-2, 5-diphenyl tetrasoliumbromide) assay. RESULTS: Newly constructed film did not show any cell cytotoxicity against human lens epithelial cells, HLE-B3. In the lysozyme-free PBS, 62% of C/Gly-1 and 53% of C/Gly-0.7 were degraded respectively after 4 weeks. CONCLUSIONS: This film was designed to be inserted between artificial crystalline and posterior capsule. With its transparency, this film may not interfere the vision of patient. This film is also completely degradable in the lysozyme containing condition. With these characteristics, this film may be used in the drug delivery system that inhibits the formation of after cataract. |
| Key Words:
After cataract;Cataract;Drug delivery system |
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