| Immunohistochemical Localization of Transglutaminase 4 in the Human Eye. |
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Yeoun Sook Chun, Seung Hee Seo, In Gyu Kim, Won Ryang Wee, Jin Hak Lee |
1Department of Ophthalmology, Seoul Veterans Hospital, Korea.
2Department of Ophthalmology, Seoul National University College of Medicine, Korea. wrwee@snu.ac.kr
3Department of Biochemistry, Seoul National University College of Medicine, Korea.
4Seoul Artificial Eye Center, Seoul National University Hospital, Clinical Research Institute, Korea. |
| 사람 눈에서 Transglutaminase 4의 발현과 분포양상 |
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전연숙 ( Jeon Yeon Sug ) , 서승희 ( Seo Seung Hui ) , 김인규 ( Kim In Gyu ) , 위원량 ( Wi Won Lyang ) , 이진희 ( Lee Jin Hui ) |
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| Abstract |
PURPOSE
Transglutaminase 4 (TGase 4) belongs to a family of enzymes that catalyzes the post-translational modification of proteins. In an attempt to establish its physiological function(s), the distribution of TGase 4 expression in the human eye was determined. METHODS: Ocular tissues obtained from five human whole eyeball postmortem (40(+1) weeks at gestation age, 2 months, 48, 66, 76 years) were stained with monoclonal antibodies against human TGase 4 using indirect immunohistochemical method. RESULTS: TGase 4 was found in the lacrimal glands, corneal epithelium and endothelium, conjunctival epithelium, lens epithelium, retina (inner segment of photoreceptor, external limiting membrane, outer plexiform layer, inner plexiform layer, retinal nerve fiber layer and internal limiting membrane), iris, ciliary muscle, ciliary nonpigmented epithelium and trabecular meshwork. Endothelium of blood vessels in all ocular tissues was also stained. Conjunctival stroma, choroid, anterior tenon's capsule were faintly stained. No evidence of immunostaining for TGase 4 was found in the corneal stroma, iris stroma, lens nucleus, ciliary process, sclera, extraocular muscle and optic nerve. CONCLUSIONS: The expression pattern of TGase 4 was different from those of other TGase isoforms in the human eye. This result may be helpful in further investigation of the role of TGase 4 in the ocular tissue. |
| Key Words:
Immunohistochemial staining;Ocular tissues;Transglutaminase 4 |
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