Journal of the Korean Ophthalmological Society 2003;44(10):2387-2396.
Published online October 1, 2003.
The Effects of Growth Factors on the Contractility of Muller Cells.
Joong Young Jeong, Shin Dong Kim, Yang Ho Sung
1Department of Ophthalmology, Kosin University College of Medicine, Korea. shdkim@ns.kosinmed.or.kr
2Yang Ho Sung Eye Center, Kimhea, Korea.
Muller세포 수축력에 미치는 성장인자의 효과
정중영 ( Jeong Jung Yeong ) , 김신동 ( Kim Sin Dong ) , 양호성 ( Yang Ho Seong )
Abstract
PURPOSE
To assess the ability of retinal Muller cells that are to generate tractional forces during culture and to evaluate their responsiveness to contraction-stimulating growth factors. METHODS: After being dissociated from porcine retina, Muller cells were cultured, and identified by immunocytochemistry. The cells were applied to the collagen gel, and changes in the thickness of the collagen layer over time were measured. Then these values were used to estimate Muller cell's contractility indirectly. Each of the applications was classified by an initial cell population and added IGF-I and PDGF concentrations. RESULTS: The contraction rate of collagen at 24 hours into incubation differed significantly between the cell groups, with group 1 having a ratio of 5.08 +/- 0.81, group 2; 7.96 +/- 0.44, group 3; 21.46 +/- 0.86, and group 4; 28.36 +/- 1.64% (p=0.000). The contraction rate of the IGF-treated groups and the PDGF-treated groups are increased by their concentrations (P<0.05), and the contraction rate of the IGF-treated groups was higher than the PDGF-treated groups at all concentration (P<0.05). CONCLUSIONS: As the cell number and concentration of growth factors were increased, the contractility of Muller cells was elevated. The development of neutralizing antibody to IGF-I and PDGF can be one of the ways for prevention of proliferative vitreoretinopathy clinically.
Key Words: Contractility;Insulin-like growth factor-I;Muller cell;Platelet-derived growth factor


ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
SKY 1004 Building #701
50-1 Jungnim-ro, Jung-gu, Seoul 04508, Korea
Tel: +82-2-583-6520    Fax: +82-2-583-6521    E-mail: kos08@ophthalmology.org                

Copyright © 2024 by Korean Ophthalmological Society.

Developed in M2PI

Close layer
prev next