Stromelysin Gene Expression in Rat Eye Mediated by the Adenovirus Vector.

Kang, Shinwook; Hur, Won Hee; Kee, Changwon

Original Article

Journal of the Korean Ophthalmological Society 2001;42(2):378-385.
Published online February 1, 2001.

Stromelysin Gene Expression in Rat Eye Mediated by the Adenovirus Vector.

Shinwook Kang, Won Hee Hur, Changwon Kee

Department of Ophthalmology, Samsung Medical Center, School of Medicine, Sungkyunkwan University.

백서 눈에서 아데노바이러스 벡터를 매개로 한 Stromelysin 유전자의 발현

강신욱(Shin Wook Kang),허원희(Won Hee Hur),기창원(Chang Won Kee)

Abstract

The purpose of this study is to evaluate the eligibility of gene therapy in treating glaucoma by constructing a replication deficient recombinant adenovirus carrying cDNA of stromelysin and by observing its expression in the rat eye. Stromelysin cDNA was obtained by RT-PCR using total RNA extracted from the cultured human trabecular cells. The cDNA was cloned into TA vector and subsequently into p deltaA. CMV. pA. The adenovirus vector containing stromelysin cDNA was constructed by co-transfection of pJM17 and p delta A. CMV-str. pA into the 293 cells. The expression of stromelysin in the trabecular meshwork and uveoscleral outflow channel of the rat eyes was detected by in situ hybridization and immunohistochemistry. In conclusion, this study showed the possibility of gene therapy in glaucoma by showing the expression of stromelysin with recombinant adenovirus vector containing stromelysin cDNA in the rat eye.

Key Words: Adenovirus vector;Gene therapy;Glaucoma;In vivo study;Stromelysin