Effect of Latanoprost on the Cultured Human Uveal Melanocytes.

Kim, Eung Cheol; Kim, Jae Woo

Original Article

Journal of the Korean Ophthalmological Society 2001;42(6):893-897.
Published online June 1, 2001.

Effect of Latanoprost on the Cultured Human Uveal Melanocytes.

Eung Cheol Kim, Jae Woo Kim

Department of Ophthalmology, Catholic University of Daegu, Daegu, Korea.

배양된 포도막 멜라닌세포에 대한 Latanoprost 의 효과

김응철(Eung Cheol Kim),김재우(Jae Woo Kim)

Abstract

PURPOSE
To investigate the effect of latanoprost on the melanogenesis and endogenous PG release. METHODS: Primarily cultured uveal human melanocytes were exposed to 0.1 micrometer, 1 micrometer, and 10 micrometer of PhXA85 and also co-exposured with 1 micrometer of indomethacine or hydrocortisone, the melanin content of each melanocyte was measured with spectrophotometer after 1 week. RESULTS: After 1 week, the melanin content of each melanocyte was increased with 1 micrometer, 10 micrometer of PhXA85(p<0.05). After co-exposure with indomethacine or hydrocortisone, the amount of increased melanin content was less than that of PhXA85 alone and statistically significant with 10 micrometer of PhXA85(p<0.05). CONCLUSIONS: These results show that PhXA85 stimulates melanogenesis of cultured human uveal mela-nocytes and also suggests the possibility of exogenous PGF2 alpha-induced release of endogenous prostaglandin.

Key Words: Hydrocortisone;Indomethacine;Latanoprost;Melanocyte