Journal of the Korean Ophthalmological Society 2002;43(7):1293-1301.
Published online July 1, 2002.
Immunohistochemical Distribution of Heme Oxygenase Isoenzymes in Developing Rat Retina.
Yang Keum Kang, Hwa Young Lee, Chung Sook Ahn
1Department of Ophthalmology, Inchon Medical Center, Seoul, Korea.
2Department of Anatomy, College of Medicine, Ewha Womans University, Seoul, Korea.
3Department of Ophthalmology, College of Medicine, Ewha Womans University, Seoul, Korea. Ewhaoph01@hanmail.net
성장기 흰쥐 망막내 Heme Oxygenases 의 분포에 관한 면역조직화학적 연구
강양금 ( Yang Keum Kang ) , 이화영 ( Hwa Young Lee ) , 안정숙 ( Chung Sook Ahn )
Abstract
PURPOSE
To analyze and compare the immunohistochemical distributions of HO-1 and HO-2 with the developmental process of retina, focusing on the time of birth and opening eyes because it is the time of the exposure to hyperoxia and light exposure. METHODS: The retina of 16-day-old embryo, 18-day-old embryo, 20-day-old embryo and postnatal rats at 0 day, 5 days, 10 days, 15 days, 20 days, 1 month, 2 months, 3 months were examined by hematoxylineeosin staining and immunohistochemistry of HO-1 and HO-2. RESULTS: Similar distribution of HO-1 and HO-2 from embryo to mature retina were observed. It was observed that HO-1 and HO-2 was contained in ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer and pigment layer of the mature retina. In the developmental process, reactivity was observed in the cell body of ganglion cells after 0 day of its birth. The reactivity was increased after 5 days of its birth, staining was observed in nerve cell and nerve fiber of ganglion cell layer, inner plexiform layer, inner segment of neuroblastic layer, pigment layer at this time. Reactivity of ganglion cell layer and pigment layer was increased intensively after 15 days of its birth and weak reactivity in some of horizontal cells and amacrine cells in inner plexiform layer was observed. The same distribution as that of mature retina was observed after 20 days of its birth. CONCLUSIONS: HO-1 and HO-2 expression was temporally and spatially correlated with the normal development of retina in rats. HO was concerned with the structure and function of retinal development by the activation of its products and with anti-oxidant mechanism on the time of birth and opening eyes.
Key Words: Heme oxygenase-1 and Heme oxygenase-2;Retinal development


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