The Effect of Nitric Oxide on Retina after Introducing into Vitreal Space in Pigmented Rabbit.

Park, Y J; Kwon, N S; Lim, C S; Yang, H N

Original Article

Journal of the Korean Ophthalmological Society 1999;40(11):3079-3087.
Published online November 1, 1999.

The Effect of Nitric Oxide on Retina after Introducing into Vitreal Space in Pigmented Rabbit.

¹Department of Ophthalmology, National Police Hospital.
²Department of Biochemistry, College of Medicine, Chung Ang University, Seoul, Korea.

유색가토의 유리체강내에 Nitric Oxide 를 주사시 망막에 미치는 영향에 대한 연구

박영진(Young Jin Park),권년수(Nyun Soo Kwon),임충식(Chung Sik Lim),양한남(Han Nam Yang)

Abstract

Nitric oxide[NO], a gas with a half-life of a few seconds, has diverse physiological functions. Present study was undertaken to find retinal toxicity of NO released by NO donor in pigmented rabbits. S-nitroso-N-acetyl-D, L-penicil-lamine[SNAP], as a NO donor, dissolved in dimethyl sulfoxide[DMSO] was injected into the vitreous of pigmented rabbits. N-acetyl-D, L-penicillamine[NAP] was also injected to the control rabbits. Electroretinogram[ERG] was taken during preinjection period, 3 hours and 6 hours after injection. Light microscopic and transmission electron microscopic results were obtained at each time interval. In SNAP treated group, a-wave amplitude of maximal combined response was reduced. In contrast, b/a ratio was increased between 3 hours and 6 hours after injection. Histologic examinations showed vacuolar degeneration and clumping and lysis of nuclei of photoreceptors. Lysosome and autophagic vacuoles were also noted in the cytoplasm of outer neuronal cells. In conjunction with above results, further studies about optimal concentration are required in order to use NO in the medical fields of ophthalmology.

Key Words: Electroretinogram;Nitric oxide;Nitric oxide synthase;Retinal toxicity;S-nitroso-N-acetyl-D;L-penicillamine