The Ultra structure of Rabbit Kerato cyte Infected by Herpes Simplex Virus.

Lee, Yoon Jung; Ko, Myung Kyoo; Kim, June Gone

Original Article

Journal of the Korean Ophthalmological Society 2000;41(1):28-33.
Published online January 1, 2000.

The Ultra structure of Rabbit Kerato cyte Infected by Herpes Simplex Virus.

Yoon Jung Lee, Myung Kyoo Ko, June Gone Kim

Department of Ophthalmology, College of Medicine, Hanyang University Hospital.

배양하에서 토끼 각막간질세포의 단순포진바이러스 감염

이윤정(Yoon Jung Lee),고명규(Myung Kyoo Ko),김중곤(June Gone Kim)

Abstract

To evaluate the ultrastructure of cornea keratocyte infected by Herpes simplex virus, the cornea was excised from enucleated rabbit eyes. Cornea epithelium and endothelium of these was removed and cultivated in culture media. After 7 days, the Kos strain of Herpes simplex virus was inoculated into the cultured cornea keratocytes and co-cultivated until the cytopathic effect occurred. Cornea keratocytes was fixed in the 3%glutaraldehyde and examined with electron microscope. The infected cells were active cells and slightly more round appearance than normal cells. They had microvillies composed of cytoplasmic process protru-sions into collagen fibers. Virus particles was transmitted into neighboring cells easily since there was no barrier function of collagen fibers that was destroyed in the condition of culture. The nuclear changes of infected cells were marked :nuclear chromatin was degraded, condensated and displaced toward the nuclear membrane. There were many virus particles in the nucleus. There were also many degenerations in the cytoplasm, destructed numerous cytoplasmic organelles and many virus particles seen in the cytoplasm. The ultrastructural findings of keratocytes co-cultivated with Herpes simplex virus were identified from our result.

Key Words: Cornea keratocytes culture;Herpes Simplex Virus;Electron microscope