Journal of the Korean Ophthalmological Society 2000;41(2):339-348.
Published online February 1, 2000.
Phagocytosis and Acid Phosphatase Activity against Candida Albicans in Cultured Rabbit Keratocyte.
Joo Heon Roh, Young Ho Hahn, Seung Wan Sohn, Do Whan Ahn, In Gun Won
1Department of Ophthalmology, Gospel Hospital, The Kosin University.
2Department of Physiology, Gospel Hospital, The Kosin University, Pusan, Korea.
3Department of Ophthalmology, College of Medicine, Inje University.
배양된 토끼 각막간질세포의 Candida albicans 에 대한 탐식능과 Acid Phosphatase 활성도 변화
노주헌(Joo Heon Roh),한영호(Young Ho Hahn),손승완(Seung Wan Sohn),안도환(Do Whan Ahn),원인건(In Gun Won)
Abstract
It has been reported that keratocytes endocytose foreign particles both in vitro and in vivo, suggesting the active participation of keratocytes in corneal wound healing and host defense mechanism. This study was conducted to investigate the phagocytosis of keratocytes against Candida albicans[C.albicans]and the intracellular response after phagocytosis. C.albicans were fixed with glutaraldehyde and then coated with fibronectin. After exposing these C.albicans to the cultured rabbit keratocytes, the phagocytosis of keratocytes against C.albicans was evaluated by light microscope[LM]and transmission electron microscope[TEM], while the intracellular response was evaluated by changes of acid phosphatase activity. Also the study about latex beads was performed at the same time to know even if keratocytes can phagocytose foreign particles, regardless of wheather or not the particles are biodegradable. After Wright staining, phagocytosed latex beads and C.albicans were observed on LM and these were recognized to be surrounded by limiting membranes inside the cytoplasm of keratocytes on TEM. The phagocytic rates of fibronectin-coated were increased to 1.5 times , as compared with that of non-coated group. Acid phosphatase activities were higher in C.albicans-exposed groups than in control[keratocytes cultured without C.albicans or latex beads]during the culture period of 24 hours and they also increased according to culture duration and reached to the plateau after 12 hours. In comparison with non-coated group, fibronectin-coated groups showed a increasing tendency of acid phosphatase activity. These results suggest that keratocytes can phagocytose not only foreign particles but also C.albicans and that fibronectin may act as effective opsonin on phagocytosis, and that keratocytes phagocytosing C.albicans increase acid phosphatase activity to digest engulfed C.albicans when corneal stroma was wounded or inflammed.
Key Words: Acid phosphatase;Candida albicans;Fibronectin;Keratocyte;Phagocytosis


ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
SKY 1004 Building #701
50-1 Jungnim-ro, Jung-gu, Seoul 04508, Korea
Tel: +82-2-583-6520    Fax: +82-2-583-6521    E-mail: kos08@ophthalmology.org                

Copyright © 2024 by Korean Ophthalmological Society.

Developed in M2PI

Close layer
prev next