Effects of Platelet-derived Growth Factor and Laminin on Retinal Pigment Epithelial Cells.

Yu, Hyeong Gon; Lee, Jaeheung; Sun, Duck Young; Chung, Hum

Original Article

Journal of the Korean Ophthalmological Society 2000;41(8):1647-1655.
Published online August 1, 2000.

Effects of Platelet-derived Growth Factor and Laminin on Retinal Pigment Epithelial Cells.

Hyeong Gon Yu, Jaeheung Lee, Duck Young Sun, Hum Chung

¹Department of Ophthalmology, Inje University Sanggye Paik Hospital, Seoul, Korea.
²Department of Ophthalmology, Seoul National University College of Medicine.
³Department of Ophthalmology, Hanil General Hospital, Seoul, Korea.

망막색소상피세포에 대한 혈소판기원성장인자와 Laminin 의 효과

유형곤(Hyeong Gon Yu),이재흥(Jae Heung Lee),선덕영(Duck Young Sun),정흠(Hum Chung)

Abstract

To investigate the effects of platelet-derived growth factor(PDGF)and laminin on the proliferation and morphological change of retinal pigment epithelial(RPE)cells, RPE cells(5 10(3))were cultured on either plastic or laminin-coated 24-well-plates for 6 days. One percent fetal calf serum and PDGF(0, 10, 20 ng/ml)were added in the culture media. Morphological change and the numbers of attached RPE cells were assessed at the end of the culture, using image analyzer and MTT assay. In image analysis, as the PDGF concentration were increased, area(A), perimeter(P), normalized perimeter(NP), diameter(D), minimum size(MinS), maximum size(MaxS), and eccentricity of binary object(EF)were increased while circle filling(Cfil)was decreased. Compared with the cells cultured on plastic, the cells on the laminin showed increase in all the parameters except EF. The proliferative effect on RPE cells were increased on both plastic and laminin as PDGF concentration were increased(P<0.05). These results suggest that proliferation and morphologic change of RPE cells might be influenced by PDGF and laminin.

Key Words: Extracellular matrix;Image analyzer;Platelet-derived growth factor;Retinal pigment epithelial cell