| Effect of Oxygen on Photoreceptor Degeneration in Retinal Degeneration Mice. |
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Mi Young Choi, Jun Ho Heo, Soo Jae Auh, Young Suk Yu |
1Department of Ophthalmology, College of Medicine, Chungbuk National University, Cheongju, Korea.
2Department of Ophthalmology, College of Medicine, Seoul National University, #28 Yeongun-dong, Chongro-ku, Seoul, 110-744, Korea. ysyu@snu.ac.kr |
| 망막변성 쥐에서 고농도의 산소가 시세포변성에 미치는 영향 |
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최미영(Mi Young Choi),어수재(Soo Jae Auh),유영석(Young Suk Yu),허준호(Jun Ho Heo) |
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| Abstract |
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There is still no effective treatment for retinal degenerative diseases, in which the loss of photoreceptor cells causes visual loss and eventually blindness. Despite the current studies show that intraocular injection of basic fibrobalst growth factor rescues damaged and dystrophic rat photoreceptor in transgenic animal, there is a few evidence that endogenous neurotrophic factor promotes photorecptor cell survival in retina. In the present study, retinal degeneration (rd)neonate mice were exposed to hyperoixa for 5 days, and then were returned to room air, which was presumed to cause relative hypoxia of nonperfused retina, producing a neovascular response. Using this model, we investigated the effect of relative hypoxia on degenerative process in rd mice. As a control, rd neonate mice were remained at room air from birth. At the postnatal 10, 12, 14, 16, 18 and 21 days, the ratio of total retinal thickness and outer nuclear layer (ONL)thickness, and neovascularization of experimental right eyes were compared with those of control. Extent of vascularization of experimental left eyes was compared with that of left eyes in control group. In rd mice, extraretinal neovascularization was observed in 62%of 93 eyes of the experimental group exposed to hyperoxia. Extent of vascularization in experimental group was smaller than that of in control. There was an oxygen-induced modulation of rates of death in experimental group, where-as the death of photoreceptor progressed in control group. In experimental group, the ratio of total retinal thickness and ONL thickness was higher than that of in control group at 18 and 21 days of age. These results suggest that the relative hypoxia in the rd mice may have rescue effect on photoreceptor in rd mice. |
| Key Words:
Neurotrophic factor;Retinal degeneration mice;Oxygen |
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