Antioxidant Activity of Xanthium Strumarium L. Extracted F ractions in Lens Epithelial Cells.

Lee, Kwang Won; Lee, Seok Jong; Park, Hyun Kyung; Kim, Myung Jin; Chung, Young Shin; Hong, Eun Kyung; Joo, Choun Ki

Original Article

Journal of the Korean Ophthalmological Society 2001;42(1):152-159.
Published online January 1, 2001.

Antioxidant Activity of Xanthium Strumarium L. Extracted F ractions in Lens Epithelial Cells.

Kwang Won Lee, Seok Jong Lee, Hyun Kyung Park, Myung Jin Kim, Young Shin Chung, Eun Kyung Hong, Choun Ki Joo

Department of Ophthalmology, Kangnam St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Korea.

수정체상피세포에서 창이자로부터 추출된 분획물들의 항산화효과

박현경(Hyun Kyung Park),이광원(Kwang Won Lee),주천기(Choun Ki Joo),이석종(Seok Jong Lee),김명진(Myung Jin Kim),정영신(Young Shin Chung),홍은경(Eun Kyung Hong)

Abstract

To elucidate the antioxidant effect of Xanthium strumarium L., a fruit of Dokomari or Daekori, which is a family of chrysanthemum on the H2O2-mediated cellualr damage, we examined the effect of Xanthium strumarium L. extraction fractions on survival of human lens epithelia, HLE B-3 cells by using cell culture system. H2O2-mediated cellualr death and its IC5 0, with approximately 100 micrometer H2O2 were determined by using MTT assay. The HLE B-3 cells pretreated with Xanthium strumarium L. extract fractions, were incubated with 100 micrometer H2O2, and in order to assess the cell viability the cultures were incubated with MTT solution. Among Xanthium strumarium L. extract fractions, Xan crude fraction, Xan CHCl3 fraction, and Xan EtAc fraction have antioxidant activity at the concentrations of 500 ng/ml, 1 microgram/ml and 100 ng/ml, respectively. These effects were statistically significant(p<0.05). Also, the fact that only the frations extracted with organic solvents have antioxidant activity suggests that the active components from the extract have hydrophobic property.

Key Words: Cataract;Lens epithelial cells;Antioxidant;Oxidative stress