Comparative Animal Study of Healon and Hyal 2000.

Choi, Si Hwan; Kil, Sook Jong; Ahn, Seung Il; Kim, Ki San

Original Article

Journal of the Korean Ophthalmological Society 1998;39(11):2612-2621.
Published online November 1, 1998.

Comparative Animal Study of Healon and Hyal 2000.

Si Hwan Choi, Sook Jong Kil, Seung Il Ahn, Ki San Kim

Department of Ophthalmology, College of Medicine, Chungnam National University.

동물실험에서 힐론과 히알 2000의 비교

최시환(Si Hwan Choi),길숙종(sook Jong Kil),안승일(Seung il Ahn),김기산(Ki San Kim)

Abstract

We compared Healon which is widely used as a viscoelastic with Hyal 2000, recently produced in Korea. We evaluated anterior chamber reaction, intraocular pressure, and specular microscopy after injection of 0.2ml Healon and Hyal 2000, respectively, into anterior chamber of 20 cats, and performed light microscopy and scanning electron microscopy 8 weeks later. We calculated the corneal swelling rate after 0.2ml Healon and Hyal 2000 were coated on the corneal endothelium of 10 rabbits and GBR solution with 0.5mM H2O2 was perfused. Anterior chamber reaction disappeared after 2 days of injections, and intraocular pressure(IOP) increased after 6 hours of infection but normalized after 24 hours in both 2 groups. Corneal endothelial cell count was 4956cells/mm2 in the Healon group and 5021cell.mm2 in the Hyal 2000 group on the first post-injection day. and 4827cells/mm2 and 4810cell/mm2, 1 week later, without a statistical difference. The histologic abnormality was not detectable in light microscopy or scanning electron microscopy in both groups. The corneal swelling rate with Healon was 15.85micrometer/hr and 12.72micrometer/hr with Hyal 2000, while it increased to 69.29micrometer/hr and 67.51micrometer/hr in controls(p<0.05), respectively. The clinical findings showed no difference and histologic findings appeared normal in both the Healon and the Hyal 2000 groups. The inhibitory effect on the corneal swelling was similar during in vitro perfusion in both groups.

Key Words: Corneal endothelial cell;Healon;Hyal 2000;Intraocular pressure