| In Vitro Amoebicidal Efficiencies of Various Disinfectants Against Four Ocular Isolates of Acanthamoeba Keratitis. |
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So Youl Kim, Hyun Hee Kong, Dong Il Chung, Tae Won Hahn |
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Department of Ophthalmology, College of Medicine, The Catholic University of Korea, St. Paul`s Hostpial. |
| 아칸토아메바 각막염에서 검출된 아칸토아메바 균주에 대한 여러 가지 소독제들의 살충효과 - 제 1 보 - |
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김소열(So Youl Kim),공현희(Hyun Hee Kong),정동일(Dong Il Chung),한태원(Tae Won Hahn) |
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| Abstract |
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To evaluate amoebicidal efficacy of various disinfectants, we treated four ocular isolates of Acanthamoeba with three kinds of commercial available contact lens(CL) disinfectants, chlorhexidine, thimerosal, polyhexamethylene biguanide(PHMB) and hexamidine, Acanthamoeba cysts were collected from axenic nonnutrient agar plates from 4-week cultures. Cyst suspension(100microliter) was inoculated on 96-well microplate with concentration of 5x103 cyssts/ml. Each well was treated with three kinds of CL disinfectants(100microliter each, 4, 8, 12, 24hrs) and two-fold diluted chlorhexidine, PHMB and hexamidine(100microliter each, 8 and 48hrs). Only one CL disinfectant containing chlorhexidine(0.005%) and thimerosal(0.001%) showed cysticidal effect. Minimal cysticidal concentration(MCC) of PHMB, chlorhexidine, hexamidine ranged 1.17-3.12microgram/ml, 3.12-12.50microgram/ml, 8.64-52.07microgram/ml respectively with 48hr treatment. In 8hr treatment, MCC of PHMB and chlorhexidine were 4.42-12.50microgram/ml and 9.31-25.0microgram/ml respectively, but hexamidine did not demonstrate cysticidal effect. Therefore for proper sterilzation of contact lens and CL caes, effective disinfectant should be included in the composition of CL cleaning solution. Among the currently used topical amoebicidal agents, PHMB was the most potent cysticidal agent in in vitro study with four ocular stains of Acanthamoeba. |
| Key Words:
Acanthamoeba;Disinfectant;Minimal cyssicidmi concentration;Polyhexamethylene biguanide |
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