| Appearance of Polymorphonuclear Leukocytes Following Corneal Scratching Wound by Impression Cytology. |
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Jin Sang Kim, Myung Kyoo Ko |
1Department of Ophthalmology Pyung-dong, 108, Chongno-gu, Seoul, Korea.
2Department of Ophthalmology, College of Medicine, Hanyang University. |
| 압입세포진단법에 의한 각막찰과상후 다형핵구의 출현 |
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김진상(Jin Sang Kim),고명규(Myung Kyoo Ko) |
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| Abstract |
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The linear scratching wound was made gently on the corneal epithelium of rabbit with 21 gauge needle under an operating microscope. Impression cytology was performed at 30 minutes, 1, 2, 3 and 4 hour and 1, 2, 3 and 4 day after 0.5% tetracaine drops under an operating microscope. The filter paper was stained with hematoxylin and eosin. At 30 minute post-scratching, a few polymorphonuclear leukocytes appeared on the scratched cornea at 3 eyes (30%). At 3 and 4 hour, numerous polymorphonuclear leukocytes with corneal epithelial cells appeared on the scratched conea. By 3 day, no inflammatory cells were shown on the filter paper in all eyes. These findings suggest that the polymorphonuclear leukocytes could infiltrate on the corneal lesion at 30 minute post-scratching and the inflammatory cells might act even on the minute corneal lesion such as corneal erosion. |
| Key Words:
Cornea;Impression cytology;Polymorphonuclear leukocyte;Scratching wound |
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