Expression of Neurofilament Protein in the Nerve of Human Cornea.

Baek, Chang Eun; Ko, Myung Kyoo; Shin, Jung Chul

Original Article

Journal of the Korean Ophthalmological Society 1998;39(6):1082-1086.
Published online June 1, 1998.

Expression of Neurofilament Protein in the Nerve of Human Cornea.

Chang Eun Baek, Myung Kyoo Ko, Jung Chul Shin

Department of Ophthalmology, College of Medicine, Hanyang University Hospital, Seoul, Korea.

인간 각막신경에서 신경미세섬유 단백의 발현

백창은(Chang Eun Baek),고명규(Myoung Kyoo Ko),신정철(Jung Chul Shin)

Abstract

This study was performed to identify the expression of neurofilament in sensory nerve of human cornea. The three normal corneal tissues were dissected. Monoclonal antibody to neurofilament protein were used to visualize the corneal nerve. The one normal cornea was examined to observe the corneal nerve with electron rnicroscope. Transmission electron rnicroscope showed that the corneal nerve contained the neurofilament within the axon. The indirect iinmunofluorescent techniques revealed the expression of neurofilament in corneal stroma. The results of this study show that immunofluorescent technique may useful method in identification of sensory nerve of human cornea, and make it easy for us to visualize corneal innervation pattern in flat section by using of antibody to neurofilament protein.

Key Words: Cornea;Electron microscope;Immunofluorescent technique;Neurofilament;Nerve