The Effects of TGF-beta2 and bFGF on the Proliferation of Retinal Pigment Epithelial Cells.

Yoon, Hee Seong; Roh, Sae Heun; Lee, Sung Chul; Jeong, Jin Hee; Yoo, Young Hyun

Original Article

Journal of the Korean Ophthalmological Society 1998;39(6):1192-1203.
Published online June 1, 1998.

The Effects of TGF-beta2 and bFGF on the Proliferation of Retinal Pigment Epithelial Cells.

Hee Seong Yoon, Sae Heun Roh, Sung Chul Lee, Jin Hee Jeong, Young Hyun Yoo

TGF-β2 및 bFGF 망막색소상피세포 증식에 미치는 영향

윤희성(Hee Seong Yoon),노세현(Sae Heun Rho),이성철(Sung Chul Lee),정진희(Jin Hee Jeong),유영현(Young Hyun Yoo)

Abstract

This study was undertaken to document the effect of transforming growth factor-beta2 (TGF-beta2(TGF-beta2) and basic fibroblast growth factor (bFGF) on the proliferation of pig retinal pigment epithelial cells (RPE). Whereas bFGF increased the proliferation, TGF-beta2 showed the inhibitory effect on the proliferation The inhibitory effect of TGF-beta2 disappeared in RPE subcultured with 10ng/ml of bFGF. Both TGF-beta2- and bFGF-specific antisense oligonucleotides blocked the autocrine effect of the growth factors. PLC-71 -specific antisense oligonucleotide inhibited the effect of TGF-beta2 and bFGF. Genistein inhibited the effect of TGF-beta2 and bFGF in dose-dependent man, ner. The data suggest the involvement. of in PLC-/1 and tyrosine kinase in signalling.

Key Words: bFGF;PLC-yl;retinal pigment epithelial cell;TGF-beta2;tyro sine kinase