The Effect of Ascorbic Acid and its Derivative on Cultured Rabbit Keratocytes.

Lee, Do Yong; Oum, Boo Sup

Original Article

Journal of the Korean Ophthalmological Society 1996;37(1):19-29.
Published online January 1, 1996.

The Effect of Ascorbic Acid and its Derivative on Cultured Rabbit Keratocytes.

¹Department of Ophthalmology, Maryknoll Hospital, Pusan, Korea.
²Department of Ophthalmology, College of Medicine, Pusan University, Pusan, Korea.

아스코르빈산과 그 유도체들이 토끼각막실질세포 배양에 미치는 효과

이도용(D Y Lee),엄부섭(B S Oum)

Abstract

The rabbit keratocytes were cultured to evaluate the effects of L-ascorbic acid(AA), ascorbic acid 2-sulfate(AA-2S), and ascorbic acid 2-phosphate(AA-2P) by measuring cell numbers and 3H-thymidine incorporation. AA at a concentration of 0.05mM enhanced the proliferation of the cells progressively. Addition of 0.1, and 0.5mM AA stimulated the proliferation of cells in the 3rd and 7th day after culture and inhibited in the 15th and 20th day in a dose-dependent manner. AA-2S showed a similar pattern to those of the AA effect, although the inhibitory effect was milder than AA. All concentrations of AA-2P enhanced the proliferation of the cells from the early phase. The effect was prominent in the late phase in a dose-dependent manner. These results suggest that AA-2P is the most stable and the least cytotoxic in the aqueous solution state or culture media and, 0.1-0.5mM concentration of it is best in the promotion of the proliferation of the cultured keratocytes.

Key Words: Ascorbic acid;Ascorbic acid 2-phosphate;Ascorbic acid 2-sulfate;Keratocytes