| Fibronectin in the Trabecular Meshwork: Immunofluorescent and Immunoeledron Microscopic Findings. |
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Young In Choi, Dong Ho Youn, Myung Kyoo Ko, Joon Kiu Choe |
1Department of Ophthalmology, Hanil General Hospital, Seoul, Korea.
2Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.
3Department of Ophthalmology, School of Medicine, Hanyang University, Seoul, Korea. |
| 섬유주에서의 Fibronectin : 면역형광 및 면역전자현미경 소견 |
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최영인(Young In Choi),윤동호(Dong Ho Youn),고명규(Myung Kyoo Ko),최준규(Joon Kiu Choe) |
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| Abstract |
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We examined the fibronectin(FN) secretion by cultured trabecular meshwork(TM) cells from a normal human eye by indirect immunofluorescent technique using mouse anti-human FN monoclonal antibody and FITC-conjugated goat anti-mouse IgG. To localize FN on frozen sections of normal TM which were obtained from 7 enucleated eyes owing to traumatic eyeball rupture, the same indirect immunofluorescent method was used. Immunoelectron microscopy was applied to demonstrate the distribution pattern of FN in the normal TM of 2 human eyes using an avidin-biotin-peroxidase complex method. In tissue culture of TM, TM cell walls and extracellular matrices showed an intense staining with antibody to FN. Indirect immunofluorescent staining of FN on frozen sections of TM showed strong positive reactions in the subendothelial region. There was no reaction in the central core of the trabecular beam. Immunoelectron microscopy revealed the reaction products to FN in the areas lining the trabecular endothelial cells. |
| Key Words:
fibronectin;immunoelectron microscopy;indirect immunofluorescence;tissue culture;trabecular meshwork |
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