J Korean Ophthalmol Soc > Volume 59(3); 2018 > Article
Journal of the Korean Ophthalmological Society 2018;59(3):252-260.
DOI: https://doi.org/10.3341/jkos.2018.59.3.252    Published online March 15, 2018.
Effects of High Glucose and Dexamethasone on the Permeability in Trabecular Meshwork Cells.
Sun Hee Kang, Jae Woo Kim
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
고포도당과 덱사메타존이 섬유주세포의 투과도에 미치는 영향
강선희⋅김재우
대구가톨릭대학교 의과대학 안과학교실
Correspondence:  Jae Woo Kim,
Email: jwkim@cu.ac.kr
Received: 15 June 2017   • Revised: 1 August 2017   • Accepted: 13 February 2018
Abstract
PURPOSE
To investigate the effects of high glucose (HG) and dexamethasone (DEX) on the survival and permeability of trabecular meshwork cells (HTMC), and associated changes in tight junctions. METHODS: Primary cultured HTMC were exposed to 5 mM low glucose (LG) or 25 mM HG with or without 1.0 µM DEX for 3 days. The permeability of the HTMC monolayer was assessed using carboxyfluorescein or transendothelial electrical resistance (TEER). Gene and protein expressions of claudin-5 and occludin were assessed with reverse transcription polymerase chain reaction (RT-PCR) and Western blot, respectively. RESULTS: HG was significantly associated with greater HTMC monolayer permeability compared to LG by both the carboxyfluorescein permeability test and TEER (p = 0.022, 0.028). HG also decreased claudin-5 and occludin mRNA expression, respectively (7.5%, 12.9%). DEX abolished HG-induced increased permeability, and increased the protein expression of claudin-5 and occludin, respectively (p = 0.015, 0.012). CONCLUSIONS: In HTMCs, DEX reversed HG-induced permeability increase. DEX increased tight junction molecules claudin-5 and occludin. Thus, DEX-induced changes in junctional proteins could be another mechanism of increased resistance through the trabecular meshwork and may result in steroid-induced glaucoma.
Key Words: Dexamethasone;High glucose;Permeability;Tight junction;Trabecular meshwork


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