J Korean Ophthalmol Soc > Volume 57(1); 2016 > Article
Journal of the Korean Ophthalmological Society 2016;57(1):113-119.
DOI: https://doi.org/10.3341/jkos.2016.57.1.113    Published online January 15, 2016.
Neuroprotective Effects of Betaxolol Mediated by Heme Oxygenase-1 Induction in RGC-5.
Jae Bong Cha, Min Young Kwon, Su Wol Chung, Je Moon Woo
1Department of Ophthalmology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. limbus68@naver.com
2Biological Sciences, University of Ulsan College of Natural Science, Ulsan, Korea.
망막신경절세포에서 헴산화효소 발현에 의한 Betaxolol의 세포보호효과
울산대학교 의과대학 울산대학교병원 안과학교실1, 울산대학교 자연과학대학 생명과학교실2
To evaluate the neuroprotective effects of betaxolol (betaxolol hydrochloride) under hypoxic conditions using retinal ganglion cells (RGC-5) and determine whether heme oxygenase-1 (HO-1) expression exerts cytoprotective effects. METHODS: In this study, cultured RGC-5 cells were incubated with different concentrations of betaxolol hydrochloride (0.1 microM, 1 microM or 5 microM) and with 10 microM zinc protoporphyrin (ZnPP), in a hypoxia incubator (1% O2, 5% CO2, 94% N2) for 48 hours and the cell viability of each group was determined. Additionally, cell viability was measured after RGC-5 cells were incubated with 5 microM of brinzolamide (Azopt(R)), brimonidine tartrate (Alphagan(R)) or travoprost (Travatan(R)). RGC-5 cells were divided into three groups and incubated under three different conditions, normoxia group (20% O2, 5% CO2), hypoxia group (1% O2, 5% CO2) and the group with 5 microM of Betoptic S(R) treated under hypoxic conditions (hypoxia, Betoptic S(R)). After incubation for 4, 8, 12 and 24 hours, HO-1 expression was analyzed using Western blotting. RESULTS: Cell viability significantly increased in RGC-5 cells treated with Betoptic S(R) compared with other antiglaucoma agents. Increased levels of HO-1 expression indicate its relevance in cell viability. Furthermore, increased RGC-5 cell viability by Betoptic S(R) was significantly reduced in the HO-1 inhibitor ZnPP-treated group. CONCLUSIONS: We reaffirmed the known cytoprotective effects of Betoptic S(R) and the results suggests that HO-1 expression exerts cytoprotective effects against hypoxia.
Key Words: Betaxolol;Heme oxygenage-1;Hypoxia;Retinal ganglion cells

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