J Korean Ophthalmol Soc > Volume 57(1); 2016 > Article
Journal of the Korean Ophthalmological Society 2016;57(1):113-119.
DOI: https://doi.org/10.3341/jkos.2016.57.1.113    Published online January 15, 2016.
Neuroprotective Effects of Betaxolol Mediated by Heme Oxygenase-1 Induction in RGC-5.
Jae Bong Cha, Min Young Kwon, Su Wol Chung, Je Moon Woo
1Department of Ophthalmology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea. limbus68@naver.com
2Biological Sciences, University of Ulsan College of Natural Science, Ulsan, Korea.
망막신경절세포에서 헴산화효소 발현에 의한 Betaxolol의 세포보호효과
차재봉1⋅권민영2⋅정수월2⋅우제문1
울산대학교 의과대학 울산대학교병원 안과학교실1, 울산대학교 자연과학대학 생명과학교실2
Abstract
PURPOSE
To evaluate the neuroprotective effects of betaxolol (betaxolol hydrochloride) under hypoxic conditions using retinal ganglion cells (RGC-5) and determine whether heme oxygenase-1 (HO-1) expression exerts cytoprotective effects. METHODS: In this study, cultured RGC-5 cells were incubated with different concentrations of betaxolol hydrochloride (0.1 microM, 1 microM or 5 microM) and with 10 microM zinc protoporphyrin (ZnPP), in a hypoxia incubator (1% O2, 5% CO2, 94% N2) for 48 hours and the cell viability of each group was determined. Additionally, cell viability was measured after RGC-5 cells were incubated with 5 microM of brinzolamide (Azopt(R)), brimonidine tartrate (Alphagan(R)) or travoprost (Travatan(R)). RGC-5 cells were divided into three groups and incubated under three different conditions, normoxia group (20% O2, 5% CO2), hypoxia group (1% O2, 5% CO2) and the group with 5 microM of Betoptic S(R) treated under hypoxic conditions (hypoxia, Betoptic S(R)). After incubation for 4, 8, 12 and 24 hours, HO-1 expression was analyzed using Western blotting. RESULTS: Cell viability significantly increased in RGC-5 cells treated with Betoptic S(R) compared with other antiglaucoma agents. Increased levels of HO-1 expression indicate its relevance in cell viability. Furthermore, increased RGC-5 cell viability by Betoptic S(R) was significantly reduced in the HO-1 inhibitor ZnPP-treated group. CONCLUSIONS: We reaffirmed the known cytoprotective effects of Betoptic S(R) and the results suggests that HO-1 expression exerts cytoprotective effects against hypoxia.
Key Words: Betaxolol;Heme oxygenage-1;Hypoxia;Retinal ganglion cells


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