J Korean Ophthalmol Soc > Volume 55(3); 2014 > Article
Journal of the Korean Ophthalmological Society 2014;55(3):416-421.
DOI: https://doi.org/10.3341/jkos.2014.55.3.416    Published online March 15, 2014.
The Effects of Topical Carbonic Anhydrase Inhibitors on Nitric Oxide Production in Trabecular Meshwork Cells.
Jeung Hum Hong, Se Eun Kim, Jae Woo Kim
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
섬유주세포에서 국소 탄산탈수효소억제제가 일산화질소의 생성에 미치는 영향에 대한 연구
홍정흠⋅김세은⋅김재우
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea
Abstract
PURPOSE
To investigate and compare the effects of topical carbonic anhydrase inhibitors on the production and expression of nitric oxide in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 10, and 100 microM dorzolamide and brinzolamide using serum-deprived media for 3 days. Production of nitric oxide was assessed with Griess assay. Expressions of eNOS mRNA were assessed with RT-PCR. RESULTS: Both dorzolamide and brinzolamide increased the production of nitric oxide eNOS activity (p < 0.05). Dorzolamide had a more potent effect than brinzolamide on the production of nitric oxide and the expression of eNOS mRNA. CONCLUSIONS: Topical carbonic anhydrase inhibitors increased the production of nitric oxide, which was accompanied by increased eNOS activity. Dorzolamide had a more potent effect than brinzolamide on the production of nitric oxide and expression of eNOS mRNA in HTMC. The increased production of nitric oxide by topical carbonic anhydrase inhibitors involves mechanisms other than carbonic anhydrase inhibition.
Key Words: Brinzolamide;Dorzolamide;Nitric oxide;Topical carbonic anhydrase inhibitor;Trabecular meshwork cells


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