| Effect of Ascorbic Acid Against the Oxidative Stress-Induced Cellular Senescence in Trabecular Meshwork Cells. |
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Jae Woo Kim, Sun Hee Kang, Keun Woo Lee |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 섬유주세포에서 아스코르빈산이 산화스트레스에 대한 세포노화에 미치는 영향 |
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김재우⋅강선희⋅이근우 |
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Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea |
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| Abstract |
PURPOSE
To investigate the effect of L-ascorbic acid (LAA) on oxidative-stress-induced cellular senescence in trabecular meshwork (TM) cells. METHODS: Primarily cultured human TM cells were exposed to 0, 10, or 100 microM hydrogen peroxide for 7 days with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. SA-beta-gal staining was performed to quantify cellular senescence. RESULTS: Hydrogen peroxide decreased cellular survival, accompanied by decreased nitric oxide (NO) production. These decreases of cellular survival and NO production were abolished by co-exposure of 100 microM LAA. Analysis of SA-beta-gal staining revealed that LAA inhibited hydrogen peroxide-induced cellular senescence by 6.8% (p < 0.05). CONCLUSIONS: LAA may have a protective effect against the oxidative-stress-induced cellular senescence in TM cells. |
| Key Words:
Ascorbic acid;Oxidative stress;Senescence;Trabecular meshwork cells |
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