J Korean Ophthalmol Soc > Volume 52(11); 2011 > Article
Journal of the Korean Ophthalmological Society 2011;52(11):1337-1343.
DOI: https://doi.org/10.3341/jkos.2011.52.11.1337    Published online November 15, 2011.
Autophagy of Human Tenon's Capsule Fibroblasts Induced by Mitomycin-C.
Channy Park, Raekil Park, Seong Kwang Ryu, Dong Wook Lee
1Department of Audiology, Nambu University, Gwangju, Korea.
2VestibuloCochlear Research Center & Department of Microbiology, Wonkwang University, Iksan, Korea.
3Department of Ophthalmology, Chonbuk National University Medical School, Jeonju, Korea. ldw@jbnu.ac.kr
마이토마이신씨에 의해 유도된 인간 테논낭 섬유아세포의 자가포식현상
박찬희1,2⋅박래길1,2⋅유성광3⋅이동욱3
Department of Audiology, Nambu University1, Gwangju, Korea VestibuloCochlear Research Center & Department of Microbiology, Wonkwang University2, Iksan, Korea Department of Ophthalmology, Chonbuk National University Medical School3, Jeonju, Korea
Abstract
PURPOSE
The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon's capsule fibroblasts (HTCFs) caused by mitomycin-C. METHODS: An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3). RESULTS: Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon's capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS. CONCLUSIONS: Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon's capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts.
Key Words: Autophagy;Fibroblast;Mitomycin-C


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