J Korean Ophthalmol Soc > Volume 51(12); 2010 > Article
Journal of the Korean Ophthalmological Society 2010;51(12):1643-1651.
DOI: https://doi.org/10.3341/jkos.2010.51.12.1643    Published online December 15, 2010.
Effects of Gel Type Artificial Tears on Human Corneal Keratocytes and Conjunctival Epithelial Cells.
Jong Soo Lee, Eun Hee Kim, Soo Jin Kim, Ji Eun Lee, Na Mi Kim
1Department of Ophthalmology, Pusan National University College of Medicine, Busan, Korea. jongsool@pusan.ac.kr
2Department of Ophthalmology, Busan St. Mary's Medical Center, Busan, Korea.
3Department of Ophthalmology, Pusan National University Yangsan Hospital, Yangsan, Korea.
겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향
이종수1ㆍ김은희2ㆍ김수진1ㆍ이지은3ㆍ김나미1
Department of Ophthalmology, Pusan National University College of Medicine1, Busan, Korea Department of Ophthalmology, Busan St. Mary’s Medical Center2, Pusan, Korea Department of Ophthalmology, Pusan National University Yangsan Hospital3, Pusan, Korea
Abstract
PURPOSE
To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. METHODS: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe(R) and Systane(R) at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. RESULTS: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe(R) and Systane(R). The LDH titers increased after Soothe(R) exposure, but showed no significant difference after Systane(R) exposure. Soothe(R) and Systane(R) treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane(R) showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe(R). CONCLUSIONS: The apoptotic responses of Soothe(R) and Systane(R) are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane(R) may be less severe than Soothe(R) at higher concentrations and longer exposure times.
Key Words: Apoptosis;Human conjunctival epithelial cell;Human corneal keratocytes;Soothe(R);Systane(R)


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