J Korean Ophthalmol Soc > Volume 50(5); 2009 > Article
Journal of the Korean Ophthalmological Society 2009;50(5):762-768.
DOI: https://doi.org/10.3341/jkos.2009.50.5.762    Published online May 15, 2009.
Comparative Analysis of the Tear Protein Expression After Photorefractive Keratectomy Using Two-Dimensional Electrophoresis.
Jong Keun Chae, Sung Pyo Park, Tae Hoon Choi
1Department of Ophthalmology, Military Manpower Administration, Seoul, Korea. sg50@lycos.co.kr
2Department of Ophthalmology, Kangdong Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea.
3Department of Ophthalmology, Nune Eye Hospital, Seoul, Korea.
이차원 전기영동법을 이용한 굴절교정레이저각막절제술 후 눈물 내 단백질의 비교 분석
채종근1, 박성표2, 최태훈3
Department of Ophthalmology, Military Manpower Administration1, Seoul, Korea, / Department of Ophthalmology, Kangdong Sacred Heart Hospital, College of Medicine, Hallym University2, Seoul, Korea / Department of Ophthalmology, Nune Eye Hospital3, Seoul, Korea
Abstract
PURPOSE
To investigate the change in the tear protein composition of patients who underwent refractive surgery. METHODS: Tear samples were collected before photorefrative keratectomy (PRK), on the first, the second, and the third postoperative day, and then a month after the operation from 40 eyes of 20 patients. These tear samples were analyzed using two-dimensional gel electrophoresis (2-DE). Matrix-associated laser desorption/ionization time of flight (MALDI-TOF) was employed for the identification of expressed proteins. Control tear samples were collected from 40 eyes of 20 healthy volunteers who had no history of ocular surgery or pathology. RESULTS: On the first postoperative day, lipocalin-1 precursor, lipocalin-1, and lysozyme were up-regulated. On the second postoperative day, serum albumin precursor and serum albumin were up-regulated. The tears collected on the third postoperative day and after 1 month had similar protein expression levels to the control group. Lipocalin 1 precursor and lysozyme were up-regulated and down-regulated after reftactive surgery, respectively. However, each protein had a different molecular weight and isopotential point. CONCLUSIONS: The tear protein composition changed uniquely in the early postoperative period, and proteins with different isopotential points were detected after PRK. We hypothesized that the healing process might influence the expression of the tear proteins.
Key Words: Matrix-associated laser desorption/ionization time of flight (MALDI-TOF);Photorefractive keratectomy (PRK);Tear proteins;Two-dimensional electrophoresis (2-DE)


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