| Role of Ascorbic Acid Against the Oxidative Stress in Trabecular Meshwork Cells. |
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Ji Yong Ryu, Jae Woo Kim |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
| 섬유주세포에서 산화스트레스에 대한 아스코르빈산의 역할 |
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류지용ㆍ김재우 |
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Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea |
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| Abstract |
PURPOSE
To investigate the role of L-ascorbic acid (LAA) on the survival and its association with nitric oxide (NO) production against hydrogen peroxide-induced oxidative stress in trabecular meshwork (TM) cells. METHODS: Primarily cultured human TM cells were exposed to hydrogen peroxide; 10, 100 micrometer for 2 days, or 1 mM single exposure with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. Flow cytometry using annexin/PI double staining was performed to evaluate apoptosis. RESULTS: Hydrogen peroxide decreased cellular survival significantly in a dose-dependent manner accompanied with decreased NO production. However cellular survival and NO production were increased significantly with co-exposure of LAA. Cellular survival and NO were highly correlated. Flow cytometric analysis revealed that LAA inhibited hydrogen peroxide-induced apoptosis. CONCLUSIONS: LAA demonstrated a cytoprotective effect against the hydrogen peroxide-induced oxidative stress accompanied with increased NO production. The cytoprotective effect of LAA may be mediated by preserving NO in TM cells. |
| Key Words:
Ascorbic acid;Hydrogen peroxide;Nitric oxide;Oxidative stress;Trabecular meshwork cells |
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