Effects of Valproic Acid on the Survival of Human Tennon's Capsule Fibroblasts.

Lee, See Eun; Kim, Jae Woo

doi:2018.59.11.1056

J Korean Ophthalmol Soc > Volume 59(11); 2018 > Article

Original Article

Journal of the Korean Ophthalmological Society 2018;59(11):1056-1061.
DOI: https://doi.org/10.3341/jkos.2018.59.11.1056 Published online November 15, 2018.

Effects of Valproic Acid on the Survival of Human Tennon's Capsule Fibroblasts.

See Eun Lee, Jae Woo Kim

Department of Ophthalmology, Daegu Catholic University School of Medicine, Daegu, Korea. jwkim@cu.ac.kr

발프로익산이 인체 테논낭 섬유아세포의 생존에 미치는 영향

이시은⋅김재우

대구가톨릭대학교 의과대학 안과학교실

Correspondence: Jae Woo Kim,
Email: jwkim@cu.ac.kr

Received: 28 February 2018 • Revised: 21 March 2018 • Accepted: 18 October 2018

Abstract

PURPOSE
To investigate the effects of valproic acid on the survival of cultured human Tenon's capsule fibroblasts (HTFBs). METHODS: Primary cultured HTFBs were exposed to 0, 0.25, 0.5, and 1.0 mM valproic acid with or without 0, 1.0, 2.5 µg/mL mitomycin C, and incubated for 5 days. Cell survival was assessed using an MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and the degree of apoptosis was assessed by flow cytometry using annexin-V/propidium iodide double staining. RESULTS: Valproic acid decreased the survival of HTFBs in a dose-dependent manner, and survival was further decreased by adding mitomycin C to valproic acid. Both valproic acid and mitomycin C induced apoptosis of HTFBs. Valproic acid induced less apoptosis than mitomycin C. CONCLUSIONS: Valproic acid decreased the cellular survival of HTFBs and induced apoptosis. The antiproliferative effects of valproic acid were further enhanced by the addition of mitomycin C.

Key Words: Apoptosis;Fibroblast;Flow cytometry;Mitomycin C;Valproic acid