Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells.

Kim, Byung Moon; Kim, Jae Woo

doi:2015.56.1.104

J Korean Ophthalmol Soc > Volume 56(1); 2015 > Article

Original Article

Journal of the Korean Ophthalmological Society 2015;56(1):104-108.
DOI: https://doi.org/10.3341/jkos.2015.56.1.104 Published online January 15, 2015.

Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells.

Byung Moon Kim, Jae Woo Kim

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr

벤잘코니움, 미토마이신 C, 덱사메타존이 섬유주세포의 스트레스에 미치는 영향

김병문⋅김재우

Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea

Abstract

PURPOSE
To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellular stress in cultured human trabecular meshwork cell (HTMC) monolayers. METHODS: HTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEX for 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532 nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was used to evaluate cellular viabilities. RESULTS: The carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002% BAC (p < 0.05). Increased permeability without decreasing cell survival occurred with 0.05 microg/mL MMC. No effect on the permeability or cell survival was observed at 0.1 or 1.0 microm DEX (p > 0.05). CONCLUSIONS: BAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of cultured HTMCs.

Key Words: Benzalkonium chloride;Carboxyfluorescein;Dexamethasone;Mitomycin C;Trabecular meshwork cells