| Suppression of VEGF and STAT3 by Lipoic acid in Experimental Diabetic Rat Retina. |
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Sun Im Yu, Hee Jung Jung, Dae Young Her, Hyun Woong Kim, Il Han Yun |
1Department of Ophthalmology, Pusan Paik Hospital, InJe University College of Medicine, Pusan, Korea. maekbak@hanmail.net
2Department of Ophthalmology, Baleunnoon Eye Clinic, Pusan, Korea.
3Department of Anatomy, Pusan Paik Hospital, InJe University College of Medicine, Pusan, Korea. |
| 실험적으로 유도된 당뇨유발 쥐 망막에서 리포산에 의한 혈관내피성장인자와 STAT3 억제효과 |
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유선임1,정희정2,허대영3,김현웅1,윤일한1 |
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Department of Ophthalmology, Pusan Paik Hospital, InJe University College of Medicine1, Pusan, Korea Department of Ophthalmology, Baleunnoon Eye Clinic2, Pusan, Korea Department of Anatomy, Pusan Paik Hospital, InJe University College of Medicine3, Pusan, Korea |
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Correspondence:
Sun Im Yu, M.D.1 |
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| Abstract |
PURPOSE
We evaluated whether lipoic acid as antioxidant could inhibit expression of VEGF and STAT3 in experimental diabetic rat retina. METHODS: Diabetes was induced chemically by injection of streptozotocin in 12 rats of 18 Sprague-Dawley rats. After induction of diabetes, lipoic acid was injected into the peritonium in 6 rats. So all rats were divided into 3 groups, normal group (n=6), diabetes mellitus (DM) group (n=6), lipoic acid treated group (n=6). The ocular tissue of the rats were collected on 8 weeks after diabetes induction. Difference of VEGF and STAT3 expression was investigated by immunohistochemistry, RT-PCR, western blot. The change of VEGF and STAT3 in lipoic acid treated group were evaluated in these experimental model. RESULTS: The VEGF and STAT3 expression was elevated in diabetic rat retina. The active form STAT3, phosphorylated STAT3 was also elevated. The VEGF and STAT3 expression in lipoic acid treated group was lower than DM group. CONCLUSIONS: The lipoic acid could inhibit the VEGF and STAT3 expression in diabetic rat retina. |
| Key Words:
Diabetic retinopathy;Lipoic acid;STAT3;VEGF |
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