| The Expression of TGF-beta Isoform mRNA in the Cataract Lens Epithelial Cell. |
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Do Hyung Kim, Jae Wook Yang, Seh Kwang Park, Sung Doo Kim, Jun Hur, Ill Han Yoon, In Hak Choi |
1Department of Ophthalmology, College of Medicine, Inje University, Busan, Korea. eyeyang@inje.ac.kr
2In Je Ophthalmology Research Foundation, Department of Microbiology, College of Medicine, Inje University, Busan, Korea.
3Classic Eye Clinic, Busan, Korea. |
| 백내장 수정체 상피세포의 변형성장인자 발현 |
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김도형1,양재욱1,박세광2,김성두3,허준1,윤일한1,최인학2 |
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Department of Ophthalmology, College of Medicine, Inje University1, Busan, Korea In Je Ophthalmology Research Foundation, Department of Microbiology, College of Medicine, Inje University2, Busan, Korea Classic Eye Clinic3, Busan, Korea |
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Correspondence:
Do-Hyung Kim, M.D.1 |
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| Abstract |
PURPOSE
Transforming growth factor (TGF)-beta is recognized as being associated with cataractogenesis. We quantitated the mRNA expression of TGF-beta isoforms in cataractous crystalline lens to determine the effect of the isoforms on cataractogenesis. METHODS: With lens epithelial cells from thirty eyes in thirty patients, the mRNA expressions of TGF-beta1, beta2 and beta3 were quantitated by real-time SYBR Green polymerase chain reaction and the results were compared according to cataract type and presence of diabetes mellitus. RESULTS: Each isoform mRNA of TGF-beta was expressed: TGF-beta3 in all 30 eyes, TGF-beta1 in 29 eyes (96.7%), with the exception being one diabetic senile cataract, and TGF-beta2 in 9 eyes. The amount of TGF-beta1 mRNA expression was significantly lower in the diabetic cataracts than in the non-diabetic cataracts (P=0.056). CONCLUSIONS: TGF-beta was associated with cataractogenesis. It is significant that the expression of TGF-beta2 mRNA was decreased in all cataracts. The decrease of TGF-beta1 mRNA expression was more meaningful in the diabetic cataracts than in the non-diabetic cataracts. |
| Key Words:
Cataract lens epithelial cell;Senile cataract;TGF-beta isoform mRNA |
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