A Study of Human Scleral Permeability using Organ Culture.

Lee, Dong Soo; Lee, Hyo Won; Kim, Jae Woo

Original Article

Journal of the Korean Ophthalmological Society 2003;44(7):1662-1669.
Published online July 1, 2003.

A Study of Human Scleral Permeability using Organ Culture.

Dong Soo Lee, Hyo Won Lee, Jae Woo Kim

Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr

기관배양을 이용한 공막투과성에 관한 연구

이동수 ( Dong Soo Lee ) , 이효원 ( Hyo Won Lee ) , 김재우 ( Jae Woo Kim )

Abstract

PURPOSE
To measure the scleral permeability according to the time and regional differences using organ-cultured scleral tissues. METHODS: After measuring hydration and cell viability, scleral permeability was measured using a Ussing apparatus and rhodamine-dextran polymers. Organ-cultured scleral tissues are incubated for 1, 2, and 3 days and its permeability was measured in each quadrant. RESULTS: There are no significant difference in hydration and viability between the organ-cultured sclera and fresh sclera. Increases in permeability are greater with the 10 kDa dextran than with the 40 or 70 kDa dextran, however the scleral permeability did not change significantly with time (p>0.05) nor with regional difference (p>0.05). CONCLUSIONS: The scleral permeability decreased with increased molecular weight of tracers but did not change according to the time- and regional differences.

Key Words: Organ culture;Rhodamine-dextran;Scleral permeability