A Comparative Study of Gene Microarray between Normal and Diabetic Keratocyte in Rat Cornea.

Lee, Jong Soo; Lee, Joo Rak; Jung, Suk Chul; Oum, Boo Sup

Original Article

Journal of the Korean Ophthalmological Society 2003;44(7):1629-1638.
Published online July 1, 2003.

A Comparative Study of Gene Microarray between Normal and Diabetic Keratocyte in Rat Cornea.

Jong Soo Lee, Joo Rak Lee, Suk Chul Jung, Boo Sup Oum

¹Department of Ophthalmology, Pusan National University College of Medicine, Pusan, Korea. jongsool@pusan.ac.kr
²Mi-Rae Ai Center Eye Clinic, Korea.

정상과 당뇨가 있는 각막실질세포의 유전자비교

이종수 ( Jong Soo Lee ) , 이주락 ( Joo Rak Lee ) , 정석철 ( Suk Chul Jung ) , 엄부섭 ( Boo Sup Oum )

Abstract

PURPOSE
To differentiate the genes that are up-regulated or down-regulated and the cellular morphology in the corneal stromal cells between normal and diabetic rat. METHODS: After isolation with trizol (GIBCO, NY, USA), labeling and purification of complementary DNA(cDNA) probes were carried out using Atlas(TM) Glass Microarrays 1.0. RESULTS: Autoradiographic analysis showed that 35 genes were up-regulated and 8 genes were down-regulated by Atlas Image(TM) Software. Up-regulated genes were as calcium/calmodulin dependent protein kinase I, proteasome 26S subunit 1, prostaglandin F receptor. Down-regulated genes were aquaporin 8, cytochrome c oxidase polypeptide Vb, glypican 1. CONCLUSIONS: There were many differential genes in hormone receptor, voltage gated ion channels, oncogenes and tumor suppressors, G protein between normal and diabetic keratocyte of rat.

Key Words: Atlas(TM) Glass Microarrays 1.0;Diabetic keratocyte;Hybridization