| The Effect of Heat Shock Protein 70.1 gene (hsp70.1) on Retinal Ganglion Cell Damage after Partial Lesion of the Optic Nerve in Mice. |
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Sang Mok Lee, Ki Ho Park, Jeong Sun Seo |
1Department of Ophthalmology, College of Medicine, Seoul National University, Korea.
2Artificial Eye Center of Clinical Research Institute, Seoul National University Hospital, Korea. kihopark@snu.ac.kr
3Department of Biochemistry, Ilchun Molecular Medicine Institute MRC, Seoul National University Seoul, Korea. |
| 생쥐 시신경 압박손상 모델에서 hsp70.1 유전자의 유무가 망막신경절세포의 생존에 미치는 영향 |
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이상목 ( Sang Mok Lee ) , 박기호 ( Ki Ho Park ) , 서정선 ( Jeong Sun Seo ) |
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| Abstract |
PURPOSE
Heat shock protein (HSP) 70 has been known to have neuroprotective effect on the retinal ganglion cells (RGCs) in vitro and in a rat glaucoma model. This study was to evaluate the inducible HSP70 expresseion in the retinal ganglion cell (RGC) after partial crush injury in mice and to determine the effect of hsp70.1 on the RGCs in the normal condition and after partial crush injury of the optic nerve. METHODS: The optic nerve was crushed by clipping optic nerve with aneurysm clip (110G) for 30 seconds. H and E staining and immunohistochemical staining for inducible HSP70 was performed in the hsp70.1 knockout mice and wild type mice. The RGC counts before crush injury were compared and the losses of RGCs after 3 weeks were compared between the two groups. Loss of RGCs was monitored as a percentage of cells decreased relative to the contralateral sham-operated eye. RESULTS: Immunohistochemical staining showed HSP70 induction in the RGC layer after optic nerve crush injury in both hsp70.1 knockout mice and wild type mice. RGC count before crush injury showed no significant difference (n=8, Mann-Whitney test), and the loss of RGCs was 31.0%+/-0.1% (mean+/-SD) in the hsp70.1 knock-out mice and 32.6%+/-0.1% in the wild type mice without any significant difference between the two groups (n=8, Mann-Whitney test). CONCLUSIONS: HSP70 was induced in the mice RGC layer by optic nerve crush injury and the hsp70.1 gene didn't affect the RGC counts in normal condition and RGC survival after optic nerve crush injury in the mouse. |
| Key Words:
Heat shock protein;hsp70.1 knock-out mice;Ganglion cell damage;Crush injury |
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