| Effect of Poly [Ethylene Glycol][PEG]Graft Polymerization onto Polymethy lmethacrylate[PMMA]on Cultured Keratocyte Adhesion. |
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Mee Kum Kim, In Suk Park, Hyung Dal Park, Won Ryang Wee, Jin Hak Lee, Ki Dong Park, Young Ha Kim |
1Department of Ophthalmology, College of Medicine, Seoul National University.
2Biomaterials Research Center, Korea Institute of Science and Technology, Seoul, Korea. |
| Polymethylmethacrylate ( PMMA ) 표면의 Poly ( Ethylene Glycol ) ( PEG ) 그라프트중합이 배양된 각막간질세포의 부착에 미치는 영향 |
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위원량(Won Ryang Wee),이진학(Jin Hak Lee),김미금(Mee Kum Kim),박인숙(In Suk Park),박형달(Hyung Dal Park),박기동(Ki Dong Park),김영하(Young Ha Kim) |
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| Abstract |
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In this study, the effect of surface modification of polymethylmethacry-late[PMMA]by grafting of poly[ethylene glycol][PEG]on cell adhesion was investigated. PMMA surface was treated with ozone and then PEG-acry-late[PEGA]was graft-polymerized. Ozone treatment of the surface was car-ried out at room temperature by applying constant flow of oxygen[4.5liter/min]and 1 bar pressure. After ozone treatment, PMMA was immersed immediately in 20 wt%aq. PEG-acrylate solutions in glass ampoules. After degassing, the ampoule was sealed and kept at 60 degrees C for 24 hours to complete the graft polymerization. PMMA surface grafted with PEG revealed the enhanced oxygen content at ESCA analysis and the decreased dynamic receding contact angles. The adhesion of keratocytes onto modified PMMA was investigated. Keratocytes[4 x105cells/milliliter ]were layered on each PMMA discs which were glued to the bottom of 24-well culture plates, and cultured in a CO2 incubator for 24 hours. The adherent cells onto the surfaces were harvested by trypsinization and counted. The mean numbers of keratocytes on untreated PMMA, PEG-grafted PMMA with 1hour ozone treatment and PEG-grafted PMMA with 2 hour were 72.5 x104 and 6.5 x104 and 7.6 x104cells respectively, and there was a significant statistical difference [p=0.002], irrespective of ozone treatment period. This result suggests that surface modification of PMMA using PEG grafting may reduce etroprosthetic membrane formation of artificial cornea. |
| Key Words:
Keratocytes;Ozone;Poly[ethylene glycol]grafting;Surface modification of PMMA |
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