The Neurons Related to Lateral Rectus Muscle in Cerebellum of Rats Using Pseudorabies Virus. |
In Young Chung, Dae Jin Lee, Ji Myong Yoo |
Department of Ophthalmology, College of Medicine, Gyeongsang National University, #92 Chilam-dong, Chinju, Gyeongsangnamdo, 660-280, Korea. |
Pseudorabies 바이러스를 이용한 흰쥐 소뇌에서 외직근 관련 신경원에 관한 연구 |
정인영(In Young Chung),이대진(Dae Jin Lee),유지명(Ji Myong Yoo) |
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Abstract |
We investigated the location of neurons related to lateral rectus muscle in the cerebellum of rats. Horseradish peroxidase (HRP) and Pseudorabies virus (PRV) were injected into right lateral rectus muscle of rats. After 84~90 hours, cardiac perfusion was performed with 4% paraformaldehyde in HRP injection group and 4% paraformaldehyde-lysine-periodate in PRV injection group. Tetramethylbenzidine (TMB) neurohistochemical stain in HRP injection group and immuno-histochemical stain in PRV injection group were performed after frozen section. HRP-reactive neuronal cells were observed in ipsilateral abducens nucleus. PRV-immunoreactive neuronal cells were observed ipsilaterally in abducens nucleus, flocculus, paraflocculus, ansiform lobule, declive, dentate nucleus, infracerebellar nucleus and y nucleus and also bilaterally in nodulus, lobulus centralis, lingula, uvula vermis and fastigial nucleus. These findings show the existence of neuronal projection from the abducens nucleus to the cerebellum. This projection could be part of nerve circuits through which the cerebellum modulates visuomotor activities. |
Key Words:
Cerebellum;Lateral rectus muscle;Pseudorabies virus |
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