The Mechanism of Tissue Injury in the Animal Model of Experimental Retinal Vein Occlusion.

Park, Seok Joon; Chung, Hum

Original Article

Journal of the Korean Ophthalmological Society 1999;40(1):154-160.
Published online January 1, 1999.

The Mechanism of Tissue Injury in the Animal Model of Experimental Retinal Vein Occlusion.

¹Department of Ophthalmology, Seoul Municipal Boramae Hospital.
²Department of Ophthalmology, Seoul National University.

망막정맥폐쇄동물모델에서 조직손상의 기전에 관한 연구

박석준 , 정흠

Abstract

We investigated the development of programmed cell death of apoptosis as a mechanism of cell death in experimental retinal vein occlusion of rats. We induced hemicentral retinal veinocclusion by photocoagulation 3 or 4 out of 6 retinal veins after infecting rose bengal intravenously.The eyes were enucleated sequentially between day one to day fourteen, and the retinas were examined after H&E and TUNEL staining. Exudative retinal detachment developed one day after vein occlusion and was reabsorbed completely by day 4. The number of ganglion cells in vein-occluded retina began to decrease one day after vein occlusion and continue to decrease until day fourteen. Many cells in ganglion cell layer and inner nuclear layer were stained positively by TUNEL staining. These results suggest that apoptosis is an important mechanism of cell death in early stage of experimental retinal vein occlusion.

Key Words: Retinal vein occlusion;Ischemia;Apoptosis;TUNEL stain