Expression of Neurofilament in Human Retinal Horizontal Cell by Immunogold Labeling.

Lee, Byung Ro; Kim, Young Taek; Ko, Myung Kyoo

Original Article

Journal of the Korean Ophthalmological Society 1998;39(12):2983-2987.
Published online December 1, 1998.

Expression of Neurofilament in Human Retinal Horizontal Cell by Immunogold Labeling.

Byung Ro Lee, Young Taek Kim, Myung Kyoo Ko

Department of Ohthalmology, College of Medicine, Kangbuk Samsung Hostpital, Sung Kyun Kwan University.

면역금표지법을 이용한 사람망막 수평세포의 신경미세섬유 발현

이병로(Byung Ro Lee),김영택(Young Taek Kim),고명규(Myoung Kyoo Ko)

Abstract

Retinal horizontal cell is second-order neuron that integrates the information from photoreceptors over large retinal areas, mediating the lateral spread of visual signals to distant retina. The Neurofilament proteins, considered as neuronal markers, have been imunolocalized to mammalian retinal horizontal cess. However, the immunolabeling of Neurofilament in human, has been focused on the studies of visual pathway and large ganglion cells The goal of this study is to see whether human retinal horizontal cells are indeed neuronal nature or glial nature by immunogold labeling for electron microscopy. The sections of 65 year-old human retina showed the expression of Neurofilament by horizontal cells, which confirms the evidence of human retinal horizontal cell as neuronal nature.

Key Words: Horizontal cell;Immunogold labeling;Neurofilament;Neuronal nature