| The Effect of Transforming Growth Factor-beta and Mannose-6-Phosphate on the Proliferation of Subconjunctival Fibroblast of Rabbit. |
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Young Ghee Lee, Jee Ho Chang, Eun Duck Kay, Young Jae Hong |
1The Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.
2Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. |
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| Abstract |
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The main cause of failure in glaucoma filtering surgery is obstruction of aqueous outflow due to subconjunctival fibrosis. Transforming growth factor-beta(TGF-beta) is known to be a growth factor for subconjunctival fibroblast. Recently, mannose-6-phosphate(M-6-P) is reported to be an inhibitor of TGF-beta activity. In this study, we evaluated the effects of TGF-betas and M-6-P on the proliferation of cultured subconjunctival fibroblast of white rabbit in vitro. Cell proliferation was determined by 3H-thymidine DNA incorporation method. TGF-beta1, 2, 3 all promoted proliferation of subconjunctival fibroblast in a concentration dependent fashion and the effect of TGF-beta1 was most prominent among 3 types. Low concentration (0.01mM) of M-6-P paradoxically increased cell proliferation, but with the concentration of 1.0mM, the inhibitory effects were varied in the range of 45% to 7%. |
| Key Words:
Glaucoma filtration surgery;Mannose-6-phosphate;subconjunctival fibroblast;Transforming growth factor-beta |
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