Expression of Extracellular Matrix, c-Ha-ras and c-myc genes in Cultured Pterygial Fibroblasts.

Kim, Kwang Soo; Kim, Sang Ha; Suh, Seong Il; Suh, Min Ho

Original Article

Journal of the Korean Ophthalmological Society 1991;32(11):929-938.
Published online November 1, 1991.

Expression of Extracellular Matrix, c-Ha-ras and c-myc genes in Cultured Pterygial Fibroblasts.

Kwang Soo Kim, Sang Ha Kim, Seong Il Suh, Min Ho Suh

¹Department of Ophthalmology, Keimmyung University School of Medicine, Daegu, Korea.
²Department of Ophthalmology, Kyungpook, National University School of Medicine, Daegu, Korea.
³Department of Microbiology, Keimyung University School of Medicin, Daegu, Korea.

배양된 익상편 섬유아세포에서 세포외 기질 및 암유전자의 발현

김광수(Kwang Soo Kim),김상하(Sang Ha Kim),서성일(Seong Il Suh),서민호(Min Ho Suh)

Abstract

Pterygium, a disease of unknown origin and pathogennesis, is a chronic condition characterized by the encroachment of triangular portion of the bulbar conjunctiva onto the cornea. We have studied the expression of extracellular matrix genes and oncogenes in cultured pterygium using Northernm dot, and slot blot hybridizations. Northern hybridization with total RNA isolated from passaged (4-8 passages) cultures demonstrated expression of genes for alpha1(I) and alpha1(III) procollagen, fibronectin, and c-Ha-ras, but no expression of gene for c-myc was observed. The pterygium exhibited significantly increased expression of alpha1(I) and alphal(III) procollagen genes when compared with normal control cells(p<0.01). And We observed there were no differences between pterygium and normal control cells in the expression of genes for fibronectin and c-Ha-ras. According to these results we thought that the causes of pterygium may be related to the increased expression of alpha1(I) and alphal(III) procollagen genes but may not be related to c-Ha-ras, c-myc, and fibronectin genes.

Key Words: Extrcelular matrix genes;c-Ha-ras;c-myc;Northern;slot;dot blotting;Pterygium