Growth and Characterization of the Uveal Melanoma in Vitro.

Ko, Myung Kyoo; Choe, Joon Kiu; Kim, Dong Seob

Original Article

Journal of the Korean Ophthalmological Society 1990;31(3):303-308.
Published online March 1, 1990.

Growth and Characterization of the Uveal Melanoma in Vitro.

Myung Kyoo Ko, Joon Kiu Choe, Dong Seob Kim

Department of Ophthalmology, School of Medicine, Hanyang University, Seoul, Korea.

포도막 흑색종양 세포의 배양시 세포학적 특성

최준규(Joon Kiu Choe),김동섭(Dong Seob Kim),고명규(Myung Kyoo Ko)

Abstract

This study was performed to observe the influence of the feeder layer on the culture of the human uveal melanoma cell. Two types of melanoma cells which were obtained from the patients with choroidal melanoma were implanted into the culture flask. The melanoma cell was seeded onto the culture dish covered with conjunctival fibroblast, Vero cell, and culture dish which did not contain the feeder layer respectively. The growth pattern of the melanoma cell was observed with phase contrast microscopy upto 30 days after seeding. There was no definite difference in growth pattern between each group. These results may indicate that the feeder layer is not an essential factor in the culture of uveal melanoma cell.

Key Words: feeder layer;fibroblast;melanoma culture;Vero cell