Effect of Prostaglandin F2alpha on the Production of Nitric Oxide in Cultured Ciliary Muscle Cells. |
Jae Woo Kim, Yoon Young Kim, Keun Hae Kim |
Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea. jwkim@cu.ac.kr |
프로스타글란딘F2α가 배양된 섬모체근세포의 산화질소 생성에 미치는 영향 |
김재우,김윤영,김근해 |
Department of Ophthalmology, Catholic University of Daegu College of Medicine, Daegu, Korea |
Correspondence:
Jae Woo Kim, M.D. |
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Abstract |
PURPOSE To investigate whether prostaglandin F2alpha (PGF2alpha) affects on the production of nitric oxide (NO) in cultured ciliary muscle (CM) cells. METHODS: Following primary culture of CM cells from porcine eyes, the cells were exposed to PGF2alpha (PhXA85, latanoprost free acid) with and without pretreatments of NO synthase inhibitor (L-NAME, N omega- Nitro-L-arginine methyl ester) and cyclooxygenase inhibitors (indomethacin and dexamethasone) for 3 days. The cellular survivals were also evaluated in serum-deprived and hypoxic conditions induced by sodium cyanide. The cellular survival and nitrite production were assessed by MTT and Griess assay, respectively. RESULTS: PGF2alpha enhanced the production of NO significantly in cultured CM cells in a dose-dependent manner, while various inhibitors abolished this effect. PGF2alpha was not cytoprotective in serum-deprived and hypoxic conditions. CONCLUSIONS: The current results suggest that PGF2alpha potentiates NO production but is not cytoprotective in CM cells. This PGF2alpha-induced NO production in CM cells may be involved in the regulation of uveoscleral outflow. |
Key Words:
Ciliary muscle cells;Nitric oxide;PGF2alpha |
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