| The Effectiveness of Pediatric Blood Culture Bottle in Endophthalmitis. |
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Kyong Ho Kim, Han Jo Kwon, Sung Who Park, Ik Soo Byon, Ji Eun Lee, Boo Sup Oum, Kyung Hwa Shin |
1Department of Ophthalmology, Pusan National University Yangsan Hospital, Pusan National University School of Medicine, Yangsan, Korea.
2Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Pusan National University School of Medicine, Yangsan, Korea.
3Department of Ophthalmology, Pusan National University Hospital, Pusan National University School of Medicine, Busan, Korea. oph97@naver.com
4Department of Ophthalmology, Pusan National University School of Medicine, Yangsan, Korea.
5Korea Junggun Eye Hospital, Busan, Korea.
6Department of Laboratory Medicine, Pusan National University Hospital, Pusan National University School of Medicine, Busan, Korea. |
| 안내염 배양에서 소아용 혈액배양병의 유용성 |
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경호1,2⋅권한조3⋅박성후3,4⋅변익수1,2⋅이지은3,4⋅엄부섭5⋅신경화6 |
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부산대학교 의학전문대학원 양산부산대학교병원 안과학교실1, 부산대학교 의학전문대학원 양산부산대학교병원 의생명융합연구소2, 부산대학교 의학전문대학원 부산대학교병원 안과학교실3, 부산대학교 의학전문대학원 안과학교실4, 정근안과병원5, 부산대학교 의학전문대학원 부산대학교병원 진단검사의학과학교실6 |
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Received: 10 April 2015 • Revised: 31 May 2015 • Accepted: 21 July 2015 |
| Abstract |
PURPOSE
To investigate the effectiveness of the pediatric blood culture bottle for vitreous sample culture in endophthalmitis patients. METHODS: All consecutive cases with clinically suspected endophthalmitis treated and cultured in our institution between January 2009 and June 2013 were included in the study. Vitreous samples were obtained by vitreous needle aspiration (tap), anterior chamber aspiration, or mechanized vitreous biopsy (vitrectomy). The samples obtained using the conventional method until August 2011 were classified as group I. Since August 2011, the BacT/Alert PF pediatric blood culture bottle (bioMerieux, Marcy l'Etoile, France) was used for culture in group II. We investigated age, gender, biopsy method, cause of infection, use of antibiotics, bacterial culture, and culture positive rate. RESULTS: Thirty-three cases were included in group I and 17 cases in group II. There was no significant difference in age, gender, sampling technique, cause of infection, and use of antibiotics between the 2 groups. The culture positive rate in group II (60.7%) was significantly higher than group I (33.3%, p = 0.032). In group II, Enterococcus feacalis was the most common pathogen (8 eyes). In group I, Streptococcus pneumoniae and Pseudomonas aeruginosa were confirmed in 3 cases. CONCLUSIONS: The pediatric blood culture bottle can be used successfully in the examination of clinically suspected endophthalmitis. The method showed higher culture positive rate compared with the conventional method. This technique is simple and maintaining a supply of fresh agar media is not necessary. |
| Key Words:
Culture;Endophthalmitis;Pediatric culture media |
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