| Effect of Glucose on the Production of Reactive Oxygen Species in Retinal Pigment Epithelial Cells. |
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Jae Hyung Lee, Jae Woo Kim |
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Department of Ophthalmology, College of Medicine, Catholic University of Daegu, Daegu, Korea. jwkim@cu.ac.kr |
| 망막색소상피세포에서 포도당이 반응성산소종의 생성에 미치는 영향 |
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이재형ㆍ김재우 |
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Department of Ophthalmology, College of Medicine, Catholic University of Daegu, Daegu, Korea |
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| Abstract |
PURPOSE
To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal pigment epithelial (RPE) cells. METHODS: ARPE-19 cells were exposed to low glucose (5 mM) and high glucose (HG, 25 mM) for three days. Additionally, 50 micrometer N-acetyl cysteine (NAC) and 100 micrometer L-arginine were co-exposed. Productions of nitric oxide (NO), ROS, and superoxide were determined by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively. RESULTS: HG increased production of NO, ROS, and superoxide. HG-induced cells increased production of superoxide and ROS but were suppressed by NAC and L-arginine (substrate for NO production) as an antioxidant. CONCLUSIONS: HG increased ROS production in RPE cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in RPE cells. |
| Key Words:
High glucose;Oxidative stress;Reactive oxygen species;Retinal pigment epithelial cells |
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